LOCUS AB003468 5350 bp DNA circular SYN 21-MAY-2003 DEFINITION Cloning vector pAP3neo DNA, complete sequence. ACCESSION AB003468 VERSION AB003468.1 GI:2656021 KEYWORDS Amp; beta-lactamase; neomycin phosphotransferase; neo. SOURCE Cloning vector pAP3neo ORGANISM Cloning vector pAP3neo artificial sequences; vectors. REFERENCE 1 AUTHORS Kobori,M., Ikeda,Y., Nara,H., Kato,M., Kumegawa,M., Nojima,H. and Kawashima,H. TITLE Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library JOURNAL Genes Cells 3 (7), 459-475 (1998) MEDLINE 98424349 PUBMED 9753427 REFERENCE 2 (bases 1 to 5350) AUTHORS Nojima,H. TITLE Direct Submission JOURNAL Submitted (30-MAY-1997) Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka University, Department of Molecular Genetics; Yamadaoka 3-1, Suita, Osaka 565-0871, Japan (E-mail:hnojima@biken.osaka-u.ac.jp, Tel:81-6-6875-3980, Fax:81-6-6875-5192) COMMENT Sequence updated (11-Nov-1997). FEATURES Location/Qualifiers source 1..5350 /organism="Cloning vector pAP3neo" /mol_type="genomic DNA" /db_xref="taxon:69403" polyA_signal 47..268 /note="SV40 late polyadenylation signal" rep_origin 572..1452 /note="ColE1-derived plasmid replication origin" gene complement(1513..2373) /gene="Amp" CDS complement(1513..2373) /gene="Amp" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="BAA23652.1" /db_xref="GI:2656022" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2504..2959 /note="phage f1 region" promoter 3290..3485 /note="SV40 early promoter" gene 3596..4390 /gene="neo" CDS 3596..4390 /gene="neo" /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="BAA23653.1" /db_xref="GI:2656023" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 4446..4638 /note="SV40 late polyadenylation signal" promoter 4788..4983 /note="SV40 early promoter" promoter 5188..5207 /note="T7 promoter" promoter 5302..5321 /note="T3 promoter" BASE COUNT 1321 a 1324 c 1405 g 1300 t ORIGIN 1 ggtaccttct gaggcggaaa gaaccagccg gatccctcga gggatccaga catgataaga 61 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 121 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 181 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 241 agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt 301 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 361 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 421 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 481 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 541 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 601 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 1021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 1081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 1141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 1201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 1261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 1321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 1381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 1441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1501 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 1561 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 1621 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 1681 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 1741 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 1801 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 1861 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 1921 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 1981 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 2041 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 2101 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 2161 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 2221 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 2281 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 2341 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 2401 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 2461 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat 2521 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 2581 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 2641 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 2701 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 2761 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 2821 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 2881 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 2941 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 3001 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 3061 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 3121 cgcgtcgacg gtatcgataa gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt 3181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 3241 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 3301 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 3361 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 3421 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 3481 gcaaaaagct cctcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt 3541 gtcttttatt tcaggtcccg gatctgatca agagacagga tgaggatcgt ttcgcatgat 3601 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 3661 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 3721 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 3781 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 3841 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 3901 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 3961 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 4021 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 4081 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 4141 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 4201 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 4261 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 4321 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 4381 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgccg 4441 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 4501 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 4561 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 4621 ggtgtgggag gttttttagc ttggctgtgg aatgtgtgtc agttagggtg tggaaagtcc 4681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 4741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 4801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 4861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 4921 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 4981 aaaaagctcc tcgaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt 5041 cttttatttc aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga 5101 tgttgccttt acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgctgcag 5161 gagctcggac cgggccctta ggacgcgtaa tacgactcac tatagggaat tcgacgtcta 5221 gatcttaagg cgcgccaagg ggttggccac gtggtaacca cggggtggct agctagggat 5281 aacagggtaa tatagcggcc gccctttagt gagggttaat ttaaatcgta cgtcgcgatt 5341 aattaaccgc //